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Highly diverged novel subunit composition of apicomplexan F-type ATP synthase identified from Toxoplasma gondii

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dc.contributor.advisor Shanmugam, Dhanasekaran
dc.contributor.author Salunke, Rahul
dc.contributor.author Mourier, Tobias
dc.contributor.author Banerjee, Manidipa
dc.contributor.author Pain, Arnab
dc.contributor.author Shanmugam, Dhanasekaran
dc.date.accessioned 2019-08-28T00:50:33Z
dc.date.available 2019-08-28T00:50:33Z
dc.date.issued 2018-07-13
dc.identifier.citation Salunke R, Mourier T, Banerjee M, Pain A, Shanmugam D (2018) Highly diverged novel subunit composition of apicomplexan F-type ATP synthase identified from Toxoplasma gondii. PLoS Biol 16(7): e2006128. en_US
dc.identifier.uri http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/4598
dc.description.abstract The mitochondrial F-type ATP synthase, a multisubunit nanomotor, is critical for maintaining cellular ATP levels. In T. gondii and other apicomplexan parasites, many subunit components necessary for proper assembly and functioning of this enzyme appear to be missing. Here, we report the identification of 20 novel subunits of T. gondii F-type ATP synthase from mass spectrometry analysis of partially purified monomeric (approximately 600 kDa) and dimeric (>1 MDa) forms of the enzyme. Despite extreme sequence diversification, key FO subunits a, b, and d can be identified from conserved structural features. Orthologs for these proteins are restricted to apicomplexan, chromerid, and dinoflagellate species. Interestingly, their absence in ciliates indicates a major diversion, with respect to subunit composition of this enzyme, within the alveolate clade. Discovery of these highly diversified novel components of the apicomplexan F-type ATP synthase complex could facilitate the development of novel antiparasitic agents. Structural and functional characterization of this unusual enzyme complex will advance our fundamental understanding of energy metabolism in apicomplexan species. en_US
dc.description.sponsorship CSIR, DST-SERB (EMR/2016/003588),Office of Sponsored Research (OSR) OCRF-2014-CRG3-2267 and BAS/1/1020-01-01). en_US
dc.format.extent 28 en_US
dc.publisher PLOS BIOLOGY en_US
dc.subject F-type ATP synthase, Apicomplexa, T. gondii, mass spectrometry. en_US
dc.title Highly diverged novel subunit composition of apicomplexan F-type ATP synthase identified from Toxoplasma gondii en_US
dc.type Article en_US
local.division.division Biochemical Sciences Division en_US
dc.description.university -- en_US


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